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rabbit anti lpar6  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti lpar6
    Rabbit Anti Lpar6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+lpar6/Anti-LPAR6+Antibody/pm40001482-56-73-77
    Average 91 stars, based on 2 article reviews
    rabbit anti lpar6 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Gintonin Binds to Reduced LPA4 Receptor Subtype in Human Cortical Neurons in Alzheimer's Disease Brains.
    Article Snippet: After rinsing with PBS, the slides were blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize nonspecific binding. .. The primary antibodies used were mouse anti-NeuN (1:500, #MAB377, EMD Millipore, Billerica, MA, USA), rabbit anti-Iba1 (1:500, 019-19741, Wako, Osaka, Japan), FITC-anti-CD11b (1:100, 101206, Bio Legend, San Diego, CA, USA), mouse antimouse GFAP (1:500, 3670S, CST, Danvers, MA, USA), rabbit anti-LPAR1 (1:500, ALR-031, Alomone labs, Jerusalem, Israel), rabbit anti-LPAR2 (1:500, ALR-032, Alomone labs), rabbit anti-LPAR3 (1:500, ab23692, Abcam, Cambrighe, UK), rabbit anti-LPAR4 (1:500, ALR-034, Alomone labs), rabbit anti-LPAR5 (1:500, ALR-035, Alomone labs), and rabbit anti-LPAR6 (1:500, ALR-036, Alomone labs). .. The secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (1:500; Invitrogen, Waltham, MA, USA).

    Article Title: Gintonin Binds to Reduced LPA4 Receptor Subtype in Human Cortical Neurons in Alzheimer’s Disease Brains
    Article Snippet: After rinsing with PBS, the slides were blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize nonspecific binding. .. The primary antibodies used were mouse anti-NeuN (1:500, #MAB377, EMD Millipore, Billerica, MA, USA), rabbit anti-Iba1 (1:500, 019-19741, Wako, Osaka, Japan), FITC-anti-CD11b (1:100, 101206, Bio Legend, San Diego, CA, USA), mouse anti-mouse GFAP (1:500, 3670S, CST, Danvers, MA, USA), rabbit anti-LPAR1 (1:500, ALR-031, Alomone labs, Jerusalem, Israel), rabbit anti-LPAR2 (1:500, ALR-032, Alomone labs), rabbit anti-LPAR3 (1:500, ab23692, Abcam, Cambrighe, UK), rabbit anti-LPAR4 (1:500, ALR-034, Alomone labs), rabbit anti-LPAR5 (1:500, ALR-035, Alomone labs), and rabbit anti-LPAR6 (1:500, ALR-036, Alomone labs). .. The secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (1:500; Invitrogen, Waltham, MA, USA).

    Expressing:

    Article Title: Gintonin Binds to Reduced LPA4 Receptor Subtype in Human Cortical Neurons in Alzheimer's Disease Brains.
    Article Snippet: After rinsing with PBS, the slides were blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize nonspecific binding. .. The primary antibodies used were mouse anti-NeuN (1:500, #MAB377, EMD Millipore, Billerica, MA, USA), rabbit anti-Iba1 (1:500, 019-19741, Wako, Osaka, Japan), FITC-anti-CD11b (1:100, 101206, Bio Legend, San Diego, CA, USA), mouse antimouse GFAP (1:500, 3670S, CST, Danvers, MA, USA), rabbit anti-LPAR1 (1:500, ALR-031, Alomone labs, Jerusalem, Israel), rabbit anti-LPAR2 (1:500, ALR-032, Alomone labs), rabbit anti-LPAR3 (1:500, ab23692, Abcam, Cambrighe, UK), rabbit anti-LPAR4 (1:500, ALR-034, Alomone labs), rabbit anti-LPAR5 (1:500, ALR-035, Alomone labs), and rabbit anti-LPAR6 (1:500, ALR-036, Alomone labs). .. The secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (1:500; Invitrogen, Waltham, MA, USA).

    Article Title: Gintonin Binds to Reduced LPA4 Receptor Subtype in Human Cortical Neurons in Alzheimer’s Disease Brains
    Article Snippet: After rinsing with PBS, the slides were blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize nonspecific binding. .. The primary antibodies used were mouse anti-NeuN (1:500, #MAB377, EMD Millipore, Billerica, MA, USA), rabbit anti-Iba1 (1:500, 019-19741, Wako, Osaka, Japan), FITC-anti-CD11b (1:100, 101206, Bio Legend, San Diego, CA, USA), mouse anti-mouse GFAP (1:500, 3670S, CST, Danvers, MA, USA), rabbit anti-LPAR1 (1:500, ALR-031, Alomone labs, Jerusalem, Israel), rabbit anti-LPAR2 (1:500, ALR-032, Alomone labs), rabbit anti-LPAR3 (1:500, ab23692, Abcam, Cambrighe, UK), rabbit anti-LPAR4 (1:500, ALR-034, Alomone labs), rabbit anti-LPAR5 (1:500, ALR-035, Alomone labs), and rabbit anti-LPAR6 (1:500, ALR-036, Alomone labs). .. The secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (1:500; Invitrogen, Waltham, MA, USA).

    Western Blot:

    Article Title: Gintonin Binds to Reduced LPA4 Receptor Subtype in Human Cortical Neurons in Alzheimer's Disease Brains.
    Article Snippet: After rinsing with PBS, the slides were blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize nonspecific binding. .. The primary antibodies used were mouse anti-NeuN (1:500, #MAB377, EMD Millipore, Billerica, MA, USA), rabbit anti-Iba1 (1:500, 019-19741, Wako, Osaka, Japan), FITC-anti-CD11b (1:100, 101206, Bio Legend, San Diego, CA, USA), mouse antimouse GFAP (1:500, 3670S, CST, Danvers, MA, USA), rabbit anti-LPAR1 (1:500, ALR-031, Alomone labs, Jerusalem, Israel), rabbit anti-LPAR2 (1:500, ALR-032, Alomone labs), rabbit anti-LPAR3 (1:500, ab23692, Abcam, Cambrighe, UK), rabbit anti-LPAR4 (1:500, ALR-034, Alomone labs), rabbit anti-LPAR5 (1:500, ALR-035, Alomone labs), and rabbit anti-LPAR6 (1:500, ALR-036, Alomone labs). .. The secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (1:500; Invitrogen, Waltham, MA, USA).

    Article Title: Gintonin Binds to Reduced LPA4 Receptor Subtype in Human Cortical Neurons in Alzheimer’s Disease Brains
    Article Snippet: After rinsing with PBS, the slides were blocked with 5% normal goat serum (NGS) in PBS for 1 h at room temperature to minimize nonspecific binding. .. The primary antibodies used were mouse anti-NeuN (1:500, #MAB377, EMD Millipore, Billerica, MA, USA), rabbit anti-Iba1 (1:500, 019-19741, Wako, Osaka, Japan), FITC-anti-CD11b (1:100, 101206, Bio Legend, San Diego, CA, USA), mouse anti-mouse GFAP (1:500, 3670S, CST, Danvers, MA, USA), rabbit anti-LPAR1 (1:500, ALR-031, Alomone labs, Jerusalem, Israel), rabbit anti-LPAR2 (1:500, ALR-032, Alomone labs), rabbit anti-LPAR3 (1:500, ab23692, Abcam, Cambrighe, UK), rabbit anti-LPAR4 (1:500, ALR-034, Alomone labs), rabbit anti-LPAR5 (1:500, ALR-035, Alomone labs), and rabbit anti-LPAR6 (1:500, ALR-036, Alomone labs). .. The secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (1:500; Invitrogen, Waltham, MA, USA).



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    Image Search Results


    Fig. 3 LPAR6 inhibits breast cancer cell proliferation in vitro. a Efficient knockdown (KD) of LPAR6 with siLPAR6 in MCF-7 cells. *p < 0.05; ****p < 0.001; ns not significant (t test). b Efficient overexpression (OE) of LPAR6 with adenovirus in SK-BR-3 cells.

    Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

    Article Title: Lysophosphatidic acid receptor 6 regulated by miR-27a-3p attenuates tumor proliferation in breast cancer.

    doi: 10.1007/s12094-021-02704-8

    Figure Lengend Snippet: Fig. 3 LPAR6 inhibits breast cancer cell proliferation in vitro. a Efficient knockdown (KD) of LPAR6 with siLPAR6 in MCF-7 cells. *p < 0.05; ****p < 0.001; ns not significant (t test). b Efficient overexpression (OE) of LPAR6 with adenovirus in SK-BR-3 cells.

    Article Snippet: The primary and secondary antibodies used are listed below: anti-LPAR6 antibody (cat# AP52517PU-N; OriGene, Rockville, MD, USA) and anti-GAPDH antibody (cat# 10,494–1-AP; Proteintech, Wuhan, China); and anti-mouse secondary antibody (cat# SA00001-1; Proteintech) and anti-rabbit secondary antibody (cat# SA00001-15; Proteintech), respectively.

    Techniques: In Vitro, Knockdown, Over Expression